With the Fed-batch regulation, the pro- or eukaryotic cells are fed on a defined and regulated basis. Up to now, the use of this procedure was restricted to large-scale production to achieve optimal biomass, respectively product yield.
Pharmaceutically relevant products, such as insulin, antibodies, and many more, are synthesised by microorganisms and cell cultures in bioreactors.
RAMOS®-Fed-batch provides fully automatic Fed-batch fermentation in shaking flasks. The parallel technology and the approved measurement procedure of the RAMOS® were expanded by an automated dosing system.
RAMOS®-Fed-batch makes controlled feeding possible and regulates the dosing rates based on the oxygen transfer rate (OTR) or respiration quotient (RQ). The cell number-independant key parameter RQ gives information on the physiological condition and is evaluated from the oxygen and carbon dioxide transfer rate (CTR) and thus allows a metabolism-dependant regulated dosing.The dosing amount is precisely recorded by the software.
The innovative FluidTrain® Dosing and Sample Drawing Technology (FTT) ensures a commingling-free dosing and sample drawing conducted through merely one tube. Consequently, it is possible to seperatly and sterilely dose two or optionally more different media into each individual shaking flask.
The combination with the RAMOS® measuring data enables the feeding of the cultures with exactly the required amount of substrate, which leads to ideal culture growth and ideal production, respectively.
Ask here for more information about full automatic Fed-batch-Dosing for RAMOS®.
